Group-specific Multiplex Pcr Identification of Canine Faecal Lactobacillus Species
نویسندگان
چکیده
A set of forty-nine Lactobacillus isolates recovered from the faecal swabs of different dog breeds viz. Pomeranian (12), Labrador (15) and mongrel/indigenous dogs (22) were subjected to Lactobacillus group-specific multiplex PCR corresponding to the 16S-23S rRNA intergenic spacer region (ISR) and its flanking 23S rRNA gene. Twenty-three (46.9%) isolates were found to be positive for Group IV; six (12.2%) isolates for Group I and four (8.1%) isolates for Group II. In our study, sixteen (32.6%) isolates could not be identified with the used primers and hence were not assigned into any group. None of the isolates were found positive for the phylogenetic Group III. Amplification of 16S-23S rRNA ISR and its flanking 23S rRNA gene in the present study identified 33 canine faecal Lactobacillus isolates into three phylogenetic groups.
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